cd161 cells (Miltenyi Biotec)

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Miltenyi Biotec cd161 cells
Cd161 Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 465 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd161+cells/NK1%2E1+Antibody%2C+anti-mouse/10__1134_slash_s1068162025603787-166-0-14
Average 96 stars, based on 465 article reviews

cd161 cells - by Bioz Stars, 2026-09

96/100 stars

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FACS:

Article Title: Possible Relationship between the HLA-DRA1 Intron Haplotype of Three Single-Nucleotide Polymorphisms in Intron 1 of the HLA-DRA1 Gene and Autoantibodies in Children at Increased Genetic Risk for Autoimmune Type 1 Diabetes.
Article Snippet: .. In brief, a https://doi.org/10.4049/immunohorizons.2200039 D ow nloaded from http://journals.aai.org/im m unohorizons/article-pdf/6/8/614/1486003/ih2200039.pdf by guest on 06 February 2024 peripheral blood sample (30 50 ml) was obtained to isolate CD191 B cells, CD161 cells, CD141CD16 monocytes, CD41 T cells, CD81 T cells, and CD161CD661 neutrophils from PBMCs and RBCs, respectively, using magnetic-associated cell sorting (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

Article Title: CD161 is a marker of all human IL-17-producing T-cell subsets and is induced by RORC.
Article Snippet: Laura Maggi , Veronica Santarlasci , Manuela Capone, Anna Peired, Francesca Frosali, Sarah Q. Crome, Valentina Querci, Massimiliano Fambrini, Francesco Liotta, Megan K. Levings, Enrico Maggi, Lorenzo Cosmi, Sergio Romagnani and Francesco Annunziato 1 Centre of Excellence DENOThe, University of Florence, Italy 2 Department of Surgery, University of British Columbia, and Immunity and Infection Research Centre, Vancouver Coastal Health Research Institute, British Columbia, Canada 3 Department of Gynecology, Perinatology and Human Reproduction, University of Florence, Italy

Immunofluorescence:

Article Title: Interleukin (IL)-1 promotes allogeneic T cell intimal infiltration and IL-17 production in a model of human artery rejection
Article Snippet: For skin grafts, mouse neutrophils were quantified by staining 8-μm sections with rat anti–mouse Gr-1 (BD), and E-selectin expression was evaluated using mouse anti–human E-selectin ( ). .. CD161 + cells in artery grafts were detected by immunofluorescence microscopy using a mouse anti–human CD161 (Miltenyi Biotec) and an Alexa Fluor 488–conjugated anti–mouse secondary antibody (Invitrogen). .. Double staining was performed with the addition of a rabbit anti–human CD3 antibody (Dako) plus an Alexa Fluor 594–conjugated secondary antibody (Invitrogen).

Microscopy:

Staining:

Article Title: Proinflammatory Phenotype of CD161+ Double-Negative T Cells
Article Snippet: Cells were stained with the following human monoclonal antibodies: anti-CD56-PE-Cy7, anti-CD3-APC, anti-CD8-FITC, and anti-CD4-PE (all from Beckman Coulter Diagnostics). .. CD161+ cells after 3 weeks of clonal expansion were also stained with anti-CD161-PE antibodies (Miltenyi Biotec), a cocktail of antibodies against human hematopoietic lineage: anti-CD2 (RPA2.10), anti-CD3 (OKT3), anti-CD14 (61D3), anti-CD16 (CB16), anti-CD19 (HIB19), anti-CD56 (TULY56), antiCD235a (HIR2), all conjugated with FITC (eBioscience), and anti-CD294(CRTH2)-APC (clone REA598, Miltenyi Biotec) or anti-CD56-PE-Cy7 and anti-γδTCR-FITC antibodies (Elabscience). .. The resulting data were processed and represented using NovoExpress Software v. 1.2.4 (ACEA Biosciences).

Purification:

Article Title: TGF-beta indirectly favors the development of human Th17 cells by inhibiting Th1 cells.
Article Snippet: Human Th17 clones and circulating Th17 cells showed lower susceptibility to the antiproliferative effect of TGF-b than Th1 and Th2 clones or circulating Th1-oriented T cells, respectively.. Accordingly, human Th17 cells exhibited lower expression of clusterin, and higher Bcl-2 expression and reduced apoptosis in the presence of TGF-b, in comparison with Th1 cells.. Umbilical cord blood naı̈ve CD161CD4 T cells, which contain the precursors of human Th17 cells, differentiated into IL-17A-producing cells only in response to IL-1b plus IL-23, even in serum-free cultures.

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